Variation in the measurement of DNA damage by comet assay measured by the ECVAGy inter-laboratory validation trial
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چکیده
Lykke Forchhammer, Clara Johansson, Steffen Loft, Lennart Möller, Roger W. L. Godschalk, Sabine A. S. Langie, George D. D. Jones, Rachel W. L. Kwok, Andrew R. Collins, Amaya Azqueta, David H. Phillips, Osman Sozeri, Maciej Stępnik, Jadwiga Palus, Ulla Vogel, Håkan Wallin, Michael N. Routledge, Catherine Handforth, Alessandra Allione, Giuseppe Matullo, João Paulo Teixeira, Solange Costa, Patrizia Riso, Marisa Porrini and Peter Møller*
منابع مشابه
Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial.
The comet assay has become a popular method for the assessment of DNA damage in biomonitoring studies and genetic toxicology. However, few studies have addressed the issue of the noted inter-laboratory variability of DNA damage measured by the comet assay. In this study, 12 laboratories analysed the level of DNA damage in monocyte-derived THP-1 cells by either visual classification or computer-...
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The increasing use of single cell gel electrophoresis (the comet assay) highlights its popularity as a method for detecting DNA damage, including the use of enzymes for assessment of oxidatively damaged DNA. However, comparison of DNA damage levels between laboratories can be difficult due to differences in assay protocols (e.g. lysis conditions, enzyme treatment, the duration of the alkaline t...
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Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...
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تاریخ انتشار 2010